trypsin是什么意思psin在线翻译读音例句

Trypsinizingandsubculturingmammaliancellsfromamonolayer

AsImajorinmolecularbiology,therearelostsofexperimentsIneedtodoevery

i月季花 lldemonstrateafundamentalexperiment,ingor

subculturingofcellsisacommonprocedurewhereincellsfromagivencultureare

divided,or“spli关于月亮的诗句思乡 t”,intonewculturesandfedwithfreshmediumtofacilitatefurther

heconceptitselfisrelativelysimple,itisquitecomplexinpr厕的拼音 actical.

Thestepsaredescribedbelow.

Topassageaplateofcell,itmeanstostartfromaprimaryculturegrownto

confluencyin6mmdishcontaining5mltissueculturemedium.

Firstofall,removeallofthemediumfromtheprimaryculturewiththepipettor.

Thenwashtheinherentcellmonolayeronceortwicewiththesmallvolumeof

37℃PBSsolutiontoremoveresidualfetalbovinethatmayinhibittheactionof

trypsinenzymethatwilladdinnextstep.

Nextaddenough徐文长传文言文翻译 warmtrypsinEDTAsolutiontotheculturesothatitcover

heplateon37℃warmingtrayforonetotwominutes.

Aftertheincubation,tapthebottomoftheplateontheflatsurfacetodislodgethe

cktheculturewithinverted盘踞的近义词 microscopetobesurethatthecellsare

roundedupwhichindicatestheyaredetachedfromthesurface.

Afterlookingthroughthescope,wringsadishincompletemediumandpipettethe

lls

do我与地坛原文全文 wnandresuspendthecellinmedium.

Afterthat,addequalvolumeofcellsuspensiontoeachofthefreshplatesthathave

2mloffreshmediumtoeachnewculture.

Fina二月花指的是什么花 lly,incubatetheplatesin37℃5%CO

2

incubator.